Catalog Number - 68322-05P

The 68322‑05P Cogent RP PFP™ HPLC Column is designed for enhanced selectivity of halogenated, aromatic, and polar compounds, delivering improved resolution where traditional C18 phases may not fully separate analytes.

This stationary phase uses a pentafluorophenyl (PFP, “F5”) ligand bonded via a four‑carbon linker to high‑purity silica with full double end‑capping, enabling multiple interaction mechanisms including π‑π interactions, dipole interactions, and hydrogen bonding. These combined effects allow for greater selectivity control and improved separation of structurally similar compounds.

The 100 Å pore size provides broad applicability across small and moderately sized molecules, making this column well suited for routine analytical work and method development.

50 mm Length – Fast Analytical Screening & Throughput

The 50 mm length is optimized for rapid separations and high‑throughput method development, enabling efficient evaluation of selectivity.

• Short run times for faster analysis

• Efficient screening of retention and resolution

• Reduced solvent consumption

✅ Use when fast screening and method development are priorities

Flow Rate & System Requirements (4.6 mm ID, 3 µm)

⚠️ Compatible with standard HPLC systems, with moderate backpressure

• Typical flow rate range: ~0.8–1.5 mL/min

• More efficient than 5 µm columns

• Higher pressure than 5 µm, but manageable on most systems

• Standard analytical detector compatibility

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose PFP (F5) for Selective Separations

Enhanced Retention for Halogenated Compounds

• Strong interaction with fluorinated and halogenated analytes

• Improved selectivity compared to C18

Multiple Interaction Mechanisms

• π‑π interactions for aromatic compounds

• Dipole and hydrogen bonding interactions

• Greater flexibility in method development

Versatile 100 Å Pore Structure

• Suitable for a wide range of analytes

• Balanced performance across applications

Cogent RP PFP™ Advantages

Fully End‑Capped Surface

• Minimizes silanol activity

• Improves peak symmetry for polar compounds

High Efficiency (3 µm Particles)

• Higher efficiency than 5 µm columns

• Sharper peaks and improved resolution

High Purity Silica

• Consistent performance across runs

• Reliable reproducibility

Typical Applications

• Halogenated compound analysis

• Aromatic compound separations

• Pharmaceutical QA/QC workflows

• Method development and screening

• USP L43 method applications

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Higher backpressure than larger particle sizes

• Use 0.2 µm filtration to maintain column performance